GREENGATE GENOMICS CASE STUDIES

Methane-Cycling Microbiome in Permafrost

Methane-Cycling Microbiome in Permafrost

Summary:

  • Taxonomic and functional profiling of methane cycling microbiomes in permafrost soil
  • Characterization of microbial diversity via 16S rRNA amplicon data anlysis
  • Evaluation of active microbial populations by targeting microorganisms contributing to greenhouse gas (CH4 and CO2) production

 

 

Project Topic:
Analysis of methane-cycling microbiome in permafrost

Project Partner:
GFZ Potsdam, UiT, CAREERI

Location:
Siberia, Svalbard, Qinghai-Tibet Plateau

Sample type:
Permafrost soils

Target Molecule:
genomic DNA

Laboratory Analysis:

16S rRNA gene and metagenomic sequencing (Illumina) of genomic DNA retrieved from the active layer and frozen permafrost, as well as thermokarst lakes and lagoons which are subject to rapid thawing.

Bioinformatic Analysis:

Quality control, demultiplexing, taxonomic classification, metagenome assembly and binning, genome quality assessment, functional annotation, metabolic pathway reconstruction, downstream analysis including network analysis, LEfSe, ordination, SEM and multiple other numeric analyses.

Summary:

Taxonomic and functional profiling revealed high diversity of methane cycling microbiome residing in permafrost soil, with heterogeneous distribution patterns in active layer and permafrost.

More details:
https://doi.org/10.1111/mec.16118
https://doi.org/10.1002/ppp.2131
https://doi.org/10.1016/j.soilbio.2017.03.007

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Methane-Cycling Microbiome in Permafrost

Taxonomic and functional profiling revealed high diversity of methane cycling microbiome residing in permafrost soil, with heterogeneous distribution patterns in active layer and permafrost. Diversity loss after excessive warming will lead to larger variability of production rate of greenhouse gas CH4 and CO2 from permafrost soils
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