GreenGate wishes to showcase its extensive and specialized bioinformatic portfolio and is actively seeking partners and clients to pilot its services. Working with us as part of a pilot project offers the unique opportunity to receive a high quality bioinformatic service. If you are interested in such a partnership we are happy to meet and discuss how to provide our bioinformatic expertise to your project in more detail.
Here we show one-page case studies to give you a nice overview of our expertise and possible projects.

Nanopore Genome Sequencing
- Evaluation of Oxford Nanopore Technologies (ONT) base calling options
- Careful execution of different computational steps from basecalling to binning of final genome.
- Some steps of this workflow have a high influence on the size and quality of the final genome.
- Read more …

Plastisphere
- Isolation and Oxford Nanopore Sequencing of six different bacterial strain isolates from plastic polluted landfill soil
- Reconstruction of metagenome assembled genomes (MAGs)
- Taxonomic classification of recovered genomes
- Functional annotation allowed the discovery of specific genes involved in polyethylene degradation (PE) degradation
- Read more …

Atacama Desert
- Evaluation of extracellular and intracellular DNA fractions in soil and rock samples from different Atacama desert locations
- 16S rRNA amplicon sequencing and reconstruction of microbial community patterns
- Identification of highly adapted microbial species to investigate their response to extreme aridity and UV exposure, and gather important insights on microbial turnover in Mars like environments.
- Read more …

Biocide Resistance
- Differential gene expression analysis of Pseudomonas biofilms in response to sodium hypochlorite
- RNA-seq and Transcriptome data analysis
- Identification of significantly up- and down regulated genes
- Reconstruction of potential resistance pathways
- Read more …

Deep Biosphere
- Reconstruction of Microbial distribution patterns across a 230m terrestrial subsurface drill core
- Application of specifically adapted laboratory and bioinformatic workflows for low biomass environments
- Amplicon and metagenomic based taxonomy analysis
- MAG recovery and annotation allowed detection and characterization of CO2 utilizing methanogenic and chemolithotrophic microorganisms
- Statistical evaluation provided insights into microbial co-occurrence patterns and bio-geo interactions.
- Read more …

Methane-Cycling Microbiome in Permafrost
- Taxonomic and functional profiling of methane cycling microbiomes in permafrost soil
- Characterization of microbial diversity via 16S rRNA amplicon data analysis
- Evaluation of active microbial populations by targeting microorganisms contributing to greenhouse gas (CH4 and CO2) production
- Read more …

Oil and Gas Ecology
- Investigation of microbial abundance and composition s patterns in oil and gas wells to identify the potential for bio-corrosion and well fouling
- 16S rRNA and metagenomic sequencing to reconstruct microbial distribution and diversity
- Assembly and annotation of metagenomic data to identify metabolic potential
- Reconstruction and characterization of draft genomes provided insights into cellular processes in oil and gas environments.
- Read more …