{"id":408,"date":"2023-06-26T17:19:07","date_gmt":"2023-06-26T17:19:07","guid":{"rendered":"https:\/\/greengate2.h2615055.stratoserver.net\/?p=408"},"modified":"2023-08-18T08:43:38","modified_gmt":"2023-08-18T08:43:38","slug":"methane-cycling-microbiome-in-permafrost","status":"publish","type":"post","link":"https:\/\/greengategenomics.com\/de\/methane-cycling-microbiome-in-permafrost\/","title":{"rendered":"Methane-Cycling Microbiome in Permafrost"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"408\" class=\"elementor elementor-408\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-731a785c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"731a785c\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-160a34d9\" data-id=\"160a34d9\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1c400aa9 elementor-widget elementor-widget-text-editor\" data-id=\"1c400aa9\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<h2 style=\"text-align: left;\"><strong>Methane-Cycling Microbiome in Permafrost<\/strong><\/h2>\n<p><span style=\"text-decoration: underline;\">Summary:<\/span><\/p>\n<ul>\n<li>Taxonomic and functional profiling of methane cycling microbiomes in permafrost soil<\/li>\n<li>Characterization of microbial diversity via 16S rRNA amplicon data anlysis<\/li>\n<li>Evaluation of active microbial populations by targeting microorganisms contributing to greenhouse gas (CH4 and CO2) production<\/li>\n<\/ul>\n<p>\u00a0<\/p>\n<p><img fetchpriority=\"high\" decoding=\"async\" class=\"alignnone wp-image-1105 size-full\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/08\/Casestudy_permafrost5.png\" alt=\"\" width=\"544\" height=\"351\" srcset=\"https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/08\/Casestudy_permafrost5.png 544w, https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/08\/Casestudy_permafrost5-300x194.png 300w\" sizes=\"(max-width: 544px) 100vw, 544px\" \/><\/p>\n<p><img decoding=\"async\" class=\"alignnone wp-image-1104 size-full\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/08\/Casestudy_permafrost6.png\" alt=\"\" width=\"621\" height=\"293\" \/><\/p>\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\" style=\"background-color: #f2f2f2;\">\n<div class=\"wp-block-column\" style=\"flex-basis: 100%;\">\n<p>\u00a0<\/p>\n<p><strong>Project Topic:<\/strong><br \/>Analysis of methane-cycling microbiome in permafrost<\/p>\n<p><strong>Project Partner:<\/strong><br \/>GFZ Potsdam, UiT, CAREERI<\/p>\n<p><strong>Location:<\/strong><br \/>Siberia, Svalbard, Qinghai-Tibet Plateau<\/p>\n<p><strong>Sample type:<\/strong><br \/>Permafrost soils<\/p>\n<p><strong>Target Molecule:<br \/><\/strong>genomic DNA<\/p>\n<p><strong>Laboratory Analysis:<\/strong><\/p>\n<p>16S rRNA gene and metagenomic sequencing (Illumina) of genomic DNA retrieved from the active layer and frozen permafrost, as well as thermokarst lakes and lagoons which are subject to rapid thawing.<\/p>\n<p><strong>Bioinformatic Analysis:<\/strong><\/p>\n<p>Quality control, demultiplexing, taxonomic classification, metagenome assembly and binning, genome quality assessment, functional annotation, metabolic pathway reconstruction, downstream analysis including network analysis, LEfSe, ordination, SEM and multiple other numeric analyses.<\/p>\n<p><strong>Summary:<\/strong><\/p>\n<p>Taxonomic and functional profiling revealed high diversity of methane cycling microbiome residing in permafrost soil, with heterogeneous distribution patterns in active layer and permafrost.<\/p>\n<p><strong>More details:<\/strong><br \/><a href=\"https:\/\/doi.org\/10.1111\/mec.16118\">https:\/\/doi.org\/10.1111\/mec.16118<\/a><br \/><a href=\"https:\/\/doi.org\/10.1002\/ppp.2131\">https:\/\/doi.org\/10.1002\/ppp.2131<\/a><br \/><a href=\"https:\/\/doi.org\/10.1016\/j.soilbio.2017.03.007\">https:\/\/doi.org\/10.1016\/j.soilbio.2017.03.007<\/a><\/p>\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<div class=\"wp-block-buttons has-custom-font-size has-small-font-size\">\n<div class=\"wp-block-button has-custom-font-size\" style=\"font-size: 16px;\"><a href=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/08\/Casestudy_permafrost.pdf\" target=\"_blank\" rel=\"noopener\">PDF DOWNLOAD<\/a><\/div>\n<\/div>\n<div class=\"wp-block-spacer\" style=\"height: 20px;\" aria-hidden=\"true\">\u00a0<\/div>\n<\/div>\n<\/div>\n\n<div class=\"wp-block-spacer\" style=\"height: 50px;\" aria-hidden=\"true\">\u00a0<\/div>\n\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Taxonomic and functional profiling revealed high diversity of methane cycling microbiome residing in permafrost soil, with heterogeneous distribution patterns in active layer and permafrost. Diversity loss after excessive warming will lead to larger variability of production rate of greenhouse gas CH4 and CO2 from permafrost soils<\/p>","protected":false},"author":1,"featured_media":1102,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-408","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-case-studies"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Methane-Cycling Microbiome in Permafrost - GreenGate Genomics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/greengategenomics.com\/de\/methane-cycling-microbiome-in-permafrost\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Methane-Cycling Microbiome in Permafrost - GreenGate Genomics\" \/>\n<meta property=\"og:description\" content=\"Taxonomic and functional profiling revealed high diversity of methane cycling microbiome residing in permafrost soil, with heterogeneous distribution patterns in active layer and permafrost. 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