{"id":402,"date":"2023-06-26T17:01:20","date_gmt":"2023-06-26T17:01:20","guid":{"rendered":"https:\/\/greengate2.h2615055.stratoserver.net\/?p=402"},"modified":"2023-09-04T08:32:03","modified_gmt":"2023-09-04T08:32:03","slug":"deep-biosphere","status":"publish","type":"post","link":"https:\/\/greengategenomics.com\/de\/deep-biosphere\/","title":{"rendered":"Deep Biosphere"},"content":{"rendered":"<div data-elementor-type=\"wp-post\" data-elementor-id=\"402\" class=\"elementor elementor-402\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-48ff04e9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"48ff04e9\" data-element_type=\"section\" data-e-type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-29ab9c18\" data-id=\"29ab9c18\" data-element_type=\"column\" data-e-type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-78055780 elementor-widget elementor-widget-text-editor\" data-id=\"78055780\" data-element_type=\"widget\" data-e-type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t<p><\/p>\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\"><p><\/p>\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\"><h2 class=\"wp-block-post-title\">Deep Biosphere<\/h2><\/div>\n<p><\/p>\n<p><\/p>\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\"><p><\/p>\n<ul class=\"wp-block-list\"><p><\/p>\n<li>Reconstruction of Microbial distribution patterns across a 230m terrestrial subsurface drill core<\/li>\n<p><\/p>\n<p><\/p>\n<li>Application of specifically adapted laboratory and bioinformatic workflows for low biomass environments<\/li>\n<p><\/p>\n<p><\/p>\n<li>Amplicon and metagenomic based taxonomy analysis<\/li>\n<p><\/p>\n<p><\/p>\n<li>MAG recovery and annotation allowed detection and characterization of CO<sub>2<\/sub>&nbsp;utilizing methanogenic and chemolithotrophic microorganisms<\/li>\n<p><\/p>\n<p><\/p>\n<li>Statistical evaluation provided insights into microbial co-occurrence patterns and bio-geo interactions<\/li>\n<p><\/p><\/ul>\n<p><\/p><\/div>\n<p><\/p><\/div>\n<p><\/p>\n<p><\/p>\n<hr class=\"wp-block-separator aligncenter has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#b0b0b0;color:#b0b0b0\">\n<p><\/p>\n<p><\/p>\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><\/p>\n<p><\/p>\n<h2 class=\"wp-block-heading\">Case Study Deep Biosphere<\/h2>\n<p><\/p>\n<p><\/p>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><\/p>\n<p><\/p>\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\" style=\"background-color:#f2f2f2\"><p><\/p>\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\"><p><\/p>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><\/p>\n<p><\/p>\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-2 is-layout-flex wp-block-gallery-is-layout-flex\"><p><\/p>\n<figure class=\"wp-block-image size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"640\" height=\"400\" data-id=\"323\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_03.png\" alt=\"\" class=\"wp-image-323\" srcset=\"https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_03.png 640w, https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_03-300x188.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/figure>\n<p><\/p>\n<p><\/p>\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"640\" height=\"400\" data-id=\"322\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_02.png\" alt=\"\" class=\"wp-image-322\" srcset=\"https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_02.png 640w, https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_02-300x188.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/figure>\n<p><\/p>\n<p><\/p>\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"640\" height=\"400\" data-id=\"321\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_01.png\" alt=\"\" class=\"wp-image-321\" srcset=\"https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_01.png 640w, https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_01-300x188.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/figure>\n<p><\/p><\/figure>\n<p><\/p>\n<p><\/p>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><\/p>\n<p><\/p>\n<p><strong>Project Topic:<\/strong><br>Microbial utilization of CO2 in the terrestrial subsurface<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>Project Partner:<\/strong><br>GFZ Deutsches Geoforschungszentrum Potsdam<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>Location:<\/strong><br>Eger Rift mofette field in the Czech Republic<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>Sample type:<\/strong><br>Sediment and rock cores, mofette and spring waters<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>Target Molecule:<br><\/strong>DNA<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>Laboratory Analysis:<\/strong><br>16S rRNA and metagenomic sequencing of rock, water and enrichment samples<br>using Illumina and Oxford Nanopore Technology<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>Bioinformatic Analysis:<\/strong><br>Basecalling, taxonomic classification, assembly and binning, genome quality<br>assessment, annotation, metabolic pathway reconstruction<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>Summary:<\/strong><br>Specifically adapted laboratory and bioinformatic workflows allowed the reconstruction of microbial distribution patterns down to a depth of 230 m across this, difficult to work with, low biomass environment. Metagenomic genome recovery allowed the detection and characterization of CO2 utilizing methangenic and chemolithotrophic mcirroganismans. Statistical evaluation provided insights into microbial co-occurrence patterns and bio-geo interactions.<\/p>\n<p><\/p>\n<p><\/p>\n<p><strong>More details:<\/strong><br><a href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2018.02787\/full\">https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2018.02787\/full<\/a><br><a href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.543260\/full\">https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2020.543260\/full<\/a><br><a href=\"https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2017.02446\/full\">https:\/\/www.frontiersin.org\/articles\/10.3389\/fmicb.2017.02446\/full<\/a><\/p>\n<p><\/p>\n<p><\/p>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><\/p>\n<p><\/p>\n<div class=\"wp-block-buttons has-custom-font-size has-small-font-size is-vertical is-content-justification-left is-nowrap is-layout-flex wp-container-core-buttons-is-layout-fa48ed0c wp-block-buttons-is-layout-flex\"><p><\/p>\n<div class=\"wp-block-button has-custom-font-size\" style=\"font-size:16px\"><a class=\"wp-block-button__link has-white-color has-vivid-green-cyan-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Casestudies_DeepBiosphere.pdf\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">PDF DOWNLOAD<\/a><\/div>\n<p><\/p><\/div>\n<p><\/p>\n<p><\/p>\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><\/p><\/div>\n<p><\/p><\/div>\n<p><\/p>\n<p><\/p>\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<p><\/p>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>","protected":false},"excerpt":{"rendered":"<p>Specifically adapted laboratory and bioinformatic workflows allowed the reconstruction of microbial distribution patterns down to a depth of 230 m across this, difficult to work with, low biomass environment. Metagenomic genome recovery allowed the detection and characterization of CO2 utilizing methangenic and chemolithotrophic mcirroganismans. Statistical evaluation provided insights into microbial co-occurrence patterns and bio-geo interactions.<\/p>","protected":false},"author":1,"featured_media":405,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-402","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-case-studies"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Deep Biosphere - GreenGate Genomics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/greengategenomics.com\/de\/deep-biosphere\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Deep Biosphere - GreenGate Genomics\" \/>\n<meta property=\"og:description\" content=\"Specifically adapted laboratory and bioinformatic workflows allowed the reconstruction of microbial distribution patterns down to a depth of 230 m across this, difficult to work with, low biomass environment. 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