{"id":310,"date":"2023-06-26T10:01:31","date_gmt":"2023-06-26T10:01:31","guid":{"rendered":"https:\/\/greengate2.h2615055.stratoserver.net\/?p=310"},"modified":"2023-06-26T15:36:42","modified_gmt":"2023-06-26T15:36:42","slug":"plastisphere","status":"publish","type":"post","link":"https:\/\/greengategenomics.com\/de\/plastisphere\/","title":{"rendered":"Plastisphere"},"content":{"rendered":"<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\"><h2 class=\"wp-block-post-title\">Plastisphere<\/h2><\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<ul class=\"wp-block-list\">\n<li><ul class=\"has-black-color has-text-color\" style=\"box-sizing: inherit; overflow-wrap: break-word; margin: 0px; padding: 0px; list-style-type: disc; font-family: &quot;Libre Franklin&quot;, &quot;Helvetica Neue&quot;, helvetica, arial, sans-serif; white-space-collapse: collapse; font-size: 17px;\"><li style=\"box-sizing: inherit;\">Isolation and Oxford Nanopore Sequencing of six different bacterial strain isolates from plastic polluted landfill soil<\/li><li style=\"box-sizing: inherit;\">Reconstruction of metagenome assembled genomes (MAGs)<\/li><li style=\"box-sizing: inherit;\">Taxonomic classification of recovered genomes<\/li><li style=\"box-sizing: inherit;\">Functional annotation allowed the discovery of specific genes involved in polyethylene degradation (PE) degradation<\/li><\/ul><\/li>\n<\/ul>\n<\/div>\n<\/div>\n\n\n\n<hr class=\"wp-block-separator aligncenter has-text-color has-alpha-channel-opacity has-background is-style-default\" style=\"background-color:#b0b0b0;color:#b0b0b0\"\/>\n\n\n\n<div style=\"height:40px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h2 class=\"wp-block-heading\">Case Study Plastisphere<\/h2>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-columns has-background is-layout-flex wp-container-core-columns-is-layout-28f84493 wp-block-columns-is-layout-flex\" style=\"background-color:#f2f2f2\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:100%\">\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-gallery has-nested-images columns-default is-cropped wp-block-gallery-1 is-layout-flex wp-block-gallery-is-layout-flex\">\n<figure class=\"wp-block-image size-large\"><img fetchpriority=\"high\" decoding=\"async\" width=\"640\" height=\"400\" data-id=\"323\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_03.png\" alt=\"\" class=\"wp-image-323\" srcset=\"https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_03.png 640w, https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_03-300x188.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"640\" height=\"400\" data-id=\"322\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_02.png\" alt=\"\" class=\"wp-image-322\" srcset=\"https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_02.png 640w, https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_02-300x188.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/figure>\n\n\n\n<figure class=\"wp-block-image size-large\"><img decoding=\"async\" width=\"640\" height=\"400\" data-id=\"321\" src=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_01.png\" alt=\"\" class=\"wp-image-321\" srcset=\"https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_01.png 640w, https:\/\/greengategenomics.com\/wp-content\/uploads\/2023\/06\/Placeholder_Image_01-300x188.png 300w\" sizes=\"(max-width: 640px) 100vw, 640px\" \/><\/figure>\n<\/figure>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong>Project Topic:<\/strong><br>Microbial degradation of polymeric substances<\/p>\n\n\n\n<p><strong>Project Partner:<\/strong><br>GFZ Deutsches Geoforschungszentrum Potsdam<\/p>\n\n\n\n<p><strong>Location:<\/strong><br>Landfill in Brandenburg<\/p>\n\n\n\n<p><strong>Sample type:<\/strong><br>plastic polluted soil<\/p>\n\n\n\n<p><strong>Target Molecule:<br><\/strong>DNA<\/p>\n\n\n\n<p><strong>Laboratory Analysis:<\/strong><br>Culturing and isolation, full length 16S rRNA and metagenomic sequencing using Oxford Nanopore long read Technology<\/p>\n\n\n\n<p><strong>Bioinformatic Analysis:<\/strong><br>Basecalling, taxonomic classification, assembly and binning, genome quality assessment, annotation, metabolic pathway reconstruction<\/p>\n\n\n\n<p><strong>Summary:<\/strong><br>Six different bacterial strains were isolated from plastic polluted landfill soil, taxonomically classified and their genomes sequenced and reconstructed. Functional Annotation allowed the discovery of specific genes involved in polyethylene degradation and the reconstruction of a potential polyethylene (PE) degradation pathway.<\/p>\n\n\n\n<p><strong>More details:<\/strong><br><a href=\"https:\/\/doi.org\/10.3390\/microorganisms9091876\">https:\/\/doi.org\/10.3390\/microorganisms9091876<\/a><\/p>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-buttons has-custom-font-size has-small-font-size is-vertical is-content-justification-left is-nowrap is-layout-flex wp-container-core-buttons-is-layout-fa48ed0c wp-block-buttons-is-layout-flex\">\n<div class=\"wp-block-button has-custom-font-size\" style=\"font-size:16px\"><a class=\"wp-block-button__link has-white-color has-vivid-green-cyan-background-color has-text-color has-background wp-element-button\" href=\"https:\/\/test.greengategenomics.com\/wp-content\/uploads\/2023\/06\/Casestudies_Plastisphere.pdf\" style=\"border-radius:10px\" target=\"_blank\" rel=\"noreferrer noopener\">PDF DOWNLOAD<\/a><\/div>\n<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:50px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>","protected":false},"excerpt":{"rendered":"<p>Six different bacterial strains were isolated from plastic polluted landfill soil, taxonomically classified and their genomes sequenced and reconstructed. Functional Annotation allowed the discovery of specific genes involved in polyethylene degradation and the reconstruction of a potential polyethylene (PE) degradation pathway.<\/p>","protected":false},"author":1,"featured_media":324,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[11],"tags":[],"class_list":["post-310","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-case-studies"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>Plastisphere - GreenGate Genomics<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/greengategenomics.com\/de\/plastisphere\/\" \/>\n<meta property=\"og:locale\" content=\"de_DE\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Plastisphere - GreenGate Genomics\" \/>\n<meta property=\"og:description\" content=\"Six different bacterial strains were isolated from plastic polluted landfill soil, taxonomically classified and their genomes sequenced and reconstructed. 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